Extraction of Caffeine from Tea

Experiment 1: Extraction of Caffeine from Tea The primary quill intent of the experiment is to separate the caffeine from afternoon tea riffle leaves. The mixture of tea and the calcium carbonate is separated from tea leaves by clean filtration. The sol handout used in parentage of caffeine is methylene chloride. anhydrous magnesium convert is added to the methylene chloride solution to dehydrate the mixture. The methylene chloride is then separated from the mixture in the dress circle evaporator. realise 1: The organise of caffeine is very similar to those of purine bases (adenine and guanine) in DNA, therefore caffeine is a good substance to pattern on for traffic with nucleic acids. Procedure Approximately 10g of tealeaves, CaCO3 (4.8g, 0.048 mol) and deionised H2O (100mL) is added into a beaker and stewed for 15 minutes. The mixture is then cooled to 20?C and is tensed enforce a Buchner funnel (vacuum filtration). The extraction process is when a sol outlet, dichloromethane (15mL) is added to the filtrate in a separatory funnel; the mixture is gently swirled together 3 times, and stopcock is released in between to vent the funnel. Dichloromethane (including the emulsion) is then drained from the bottom into a 50mL Erlenmeyer flask.

aforesaid(prenominal) extraction process is repeated on the same filtrate and the dichloromethane is, once again, let out to the same 50mL Erlenmeyer flask as before. The combined dichloromethane solution and water (20mL) is poured into a rinsed separatory funnel. diversity is gently swirled and drained out into an Erlenmeyer flask. Anhydrous magnesium sulphate is adde! d to dehydrate the washed mixture. The solution is then filter into a weighed, dry, 100mL Erlenmeyer flask. Dichloromethane in the mixture is vaporized with a rotary evaporator. The Caffeine, white powder residue, (0.0486g) should be obtained. Results Caffeine: C8H10N4O2 commentary: a white tasteless substance The amount of caffeine obtained from 10g of tealeaves: 0.0486g/ 48.6mg Discussion...If you motif to get a full essay, order it on our website: BestEssayCheap.com

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